Pipetting technique pdf files

Five pipetting techniques to boost your performance the following tips and tricks are a short excerpt of one of the many topics covered in our gpp seminars and elearnings. Pipetting technique guide transferring liquid with a micropipette 1. Pipetting, ergonomics and you 4 ly perform the pipette cycle may affect their accuracy and precision. This is the most frequently used technique in the clinical lab. Drain a pipette with tip against the inner wall of the. Pipetting calibration and technique how they affect. Use these good pipetting practicetm techniques to improve your pipetting performance.

How to combine pdf files into one document lifewire. Impact of pipetting techniques on precision and accuracy. Pipetting accuracy is typically not decided by the brand of pipette you purchase. Air displacement pipettes are meant for general use with aqueous solutions. How to pipette the essential guide to good technique. To ensure that all users of the wjhcpor can safely use the pipettes to manipulate potentially infectious biospecimens and. Positive displacement pipettes operate very similarly to air displacement pipettes. Bacteria represent the most widely distributed and metabolically diverse group. The pipetting cycle good pipetting technique youtube. Entire volume of liquid aspirated into the pipette tip is dispensed. Avoid hurrying or rapid operation and get into a rhythm for each step in the pipetting cycle. More accurate and precise results than reverse pipetting. For standard solutions including buffers, water, dilute saline, and dilute acids and bases.

Use standard mode pipetting choose standard mode pipetting rather than reverse mode, for all but viscous samples, if accurate and precise results are desired. While mechanical pipettes are only suitable for forward and reverse pipetting, electronic dispensing systems frequently cover all of the above functions. This comprehensive good laboratory pipetting glp guide will. Choosing the correct pipetting technique helps securing the accuracy and precision necessary for reproducible, reliable results. Residual serum can sometimes be found on the outer surface of the tip. If this is a new customer, a customer information file. Tips to improve precision and data integrity precise pipetting is crucial to experimental reproducibility and. Adequate sampling technique helps to ensure that sample quality measurements are an accurate and precise estimate of the quantity of the population.

Good laboratory pipetting thermo fisher scientific. Pipetting technique training should educate attendees on the dos and donts of pipetting and how minor physical tweaks can drastically change the volume dispensed. Pipettes and pipetting aspirating the liquid steps dispensing the liquid step 4. Take a sterile plastic serological pipette also called a. Introduction to microbiology, sterile technique and. Especially when pipetting small volumes the influence of the pipetting technique can have tremendous effects on the experimental result. Henderson centre for patientoriented research wjhcpor. A good reference for safety measures can be found at artel. Place the tip just under the surface of the liquid 23 mm and smoothly release the. In positive displacement pipetting, the piston is in direct contact with the liquid. The technique is mainly recommended for aqueous solutions, such as buffers, or diluted acids or alkalis.

Pipetting techniques rainin gpp good pipetting practice. Customer information is checked for any discrepancies from that on file and will be corrected, if. Reverse pipetting also helps when pipetting ultramicro samples of 0. Impact of pipetting techniques on precision and accuracy kornelia ewald, eppendorf ag, hamburg, germany liquid handling 2 i no 020 userguide the dispensing techniques used most frequently are forward pipetting, reverse pipetting, dispensing, sequential dispensing and diluting. Customer information is checked for any discrepancies from that on file and will. The argument for pipetting technique training artel. Manual stepper pipettes are ideal for simple repetitive tasks with a.

With regards to pipetting of, for example, viscous liquids, mainly slow pipetting speed, as well as reverse pipetting. Jul 08, 2008 laboratory technique for using a volumetric pipet. Selection of the dispensing technique suitable in each case may have a significant impact on the analysis. Follow these steps for improved pipetting technique and reliable results. Once files have been uploaded to our system, change the order of your pdf documents. Pipetting techniques pdf 8 tips to improve your pipetting technique. These include proper care of the pipette, routine calibrations and development of a skill level that is associated with correct technique. How to implement pipetting technique training to improve. Organize the lab bench to allow plenty of room and work quickly. The volumetric pipet and pipetting technique youtube. A smooth rhythm will increase your throughput while minimizing errors having a timed, audible prompt can assist in. Tips to improve pipetting technique and results cappmaestro pipettes are designed for optimal use with capp expellplus pipette tips.

Introduction to microbiology, sterile technique and micropipetting. Because pipetting is a highly technique dependent activity, especially with low volume pipettes, any change in a persons ability to repeated pipetting, ergonomics and you 4 ly perform the pipette cycle may affect their accuracy and precision. Forward pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. Two main techniques exist and each should be applied depending on the sample liquid. All uploads and downloads are deemed secure and files are permanently deleted from the smallpdf servers within an hour. Both pipette types have a piston that moves in a cylinder or capillary. The technique is also recommended for dispensing very small volumes. Best practices for pbmc processing duke university. If reverse pipetting technique is used the pipette might need recalibration.

Be able to operate a pipette using the forward pipetting technique. The forward technique is recommended for aqueous solutions, such as buffers, diluted acids or alkalis. With pipetting technique training programs, pre and posttraining skills assessment are necessary to gauge improvement in pipetting accuracy and precision. In this short video, we demonstrate the five distinct steps of pipetting and how. Reverse technique the reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency to foam easily. Thermo scientific pipetting guide recommendations for pipetting different compounds solution compound examples pipette tip pipetting technique comments aqueous solution buffers, diluted salt solutions air displacement standard forward viscous solution protein and nucleic acid solutions, glycerol, tween 20406080 air displacement positive. The pdf files of the user guides are installed with the vworks software and are on the software cd that is supplied with the product. Periodically, improvements and revisions are made to product inserts. Oct 23, 2014 pipetting with an airdisplacement pipette is a five step process that is often referred to as forward pipetting. Reverse pipetting is only possible with air displacement pipettes. Reverse pipetting should be used if high accuracy is needed. Troemner is pleased to offer the pipette standards handbook to help clarify misconceptions surrounding proper pipetting techniques, operation, and calibration of these instruments.

In forward pipetting, a desired set volume of liquid is aspirated into the disposable tip and then delivered into the new container. A sponsored supplement to science your practical guide to. Techniques correct angle incorrect angle immersion angle keeping the immersion angle close to vertical can improve accuracy by up to 2. Platestreaking technique 15 minutes as with sterile pipetting, plan out manipulations before beginning to streak plates. Prerinse the tip several times before use when pipetting at temperature different from ambient. As part of our good pipetting practice program, mettler toledo has developed a. Pdf merge combinejoin pdf files online for free soda pdf. Use a consistent pipetting rhythm from sample to sample. Wipe the tip against the edge of the vessel to remove excess liquid outside the tip before dispensing.

Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. Mastering the technique means that you will be able to repeat. Correct pipetting technique for small volumes of reagents is an essential skill for researchers performing almost any type of molecular biology experiment. And when you want to do more, subscribe to acrobat pro dc. Fill a clean reagent reservoir with the liquid to be dispensed. Operator technique and laboratory environment are two critical variables that determine how a pipette will perform when used on your bench top. To ensure precise and accurate pipetting results volume in.

Technique most end users have a tendency to believe that the volume delivery is completely dependent on the setting of the micrometer dial. It uses a blowout function to ensure complete delivery of the liquid. Push the piston down to the purge position the second stop, then draw the liquid up. Obviously, this is not the case, since many factors associated with pipettes. Measurement accuracy is actually based more on the skills and techniques. Once you merge pdfs, you can send them directly to your email or download the file. This elisa technical guide will increase your awareness of elisa techniques and help you maintain proficiency with this methodology. Best practices for pbmc processing 2015 actg network annual meeting presented by. A few physical factors such as altitude, temperature, and density of liquid can influence pipetting accuracyeven if your pipetting technique is perfectand that is something you should keep in mind. Select an appropriate pipette for the volume you need to transfer all pipettes have a range in which they work, using a micropipette to transfer. By sampling only a fraction of the population, a quality estimate can be obtained more quickly and with less expense and personnel time than if the total population were measured. Introduction to microbiology, sterile technique and micropipetting background. A pdf viewer is required to open a user guide in pdf.

Due to the increased vapour pressure of methanol we recommend to prewet at least 3 times and perform reverse pipetting technique. Pipetting 3600 how you pipette directly affects the success and repeatability of your experiments. Check your product insert for specific instructions for each assay you perform. The technique is mainly recommended for solutions with a high viscosity or a tendency to foam. Self check and external calibration not only is operator technique. Northwest association for biomedical research updated july 19, 2012 1 wet lab introduction to micropipetting. If using an adjustable micropipettor, select desired volume. Use the appropriate pipette tip size from a high quality manufacturer. Choosing the correct pipetting technique helps securing the accuracy and.

Revisiting the micropipetting techniques in biomedical sciences. There is nothing wrong with that approachthey are, indeed, highly accurate and used for standard pipetting applications. While pipetting the pipette should be vertically straight the tip should be dipped only a few millimeters into the liquid. Sop consultation, training and approval print names and enter signatures and dates to certify that the persons named in this section have been consultedtrained in relation to the implementation of this. In case of solutions with a high viscosity or a tendency to foam, reverse pipetting is more suitable. This standard operating procedure sop describes the proper pipetting technique to reduce the generation of aerosols while working in the w. These five simple techniques alone can have a big impact on your results, but there are a few more to consider.

When pipetting and mixing a sample or reagent into another liquid. Tips to improve pipetting technique additional functions. Only with adobe acrobat reader you can view, sign, collect and track feedback, and share pdfs for free. Rearrange individual pages or entire files in the desired order. Follow the manufacturers guidelines for the devices you are using. Thermo scientific pipetting guide recommendations for pipetting different compounds solution compound examples pipette tip pipetting technique comments aqueous solution buffers, diluted salt. Convenient 96well format for filling microplates with a pipetman multichannel and colorcoded for easy. Capp pipettes are designed for optimal use with capp expell pipette tips.

Before you combine the files into one pdf file, use merge pdf to draganddrop pages to reorder or to delete them as you like. Self check and external calibration not only is operator technique important, but the physical capability of the pipette should be. Tips to improve the pipetting technique and results use the appropriate pipette tip size from a high quality manufacturer. Most commonly used pipetting technique that expels an exact volume of liquid from the pipette tip repeatedly. All of these factors affect precision and accuracy. This technique is intended for repeated pipetting of the same volume.

Good laboratory pipetting guide pipette calibration. For example, pain may force a scientist to sporadically change their pipetting rhythm, the pressure they apply to the pipette plunger, or their ability to draw and deliver a complete sample. Environmental factors that affect pipetting volumes include. Use this technique when pipetting viscous liquids or volatile solvents.

A smooth rhythm will increase your throughput while minimizing errors having a timed, audible prompt can assist in your accuracy. A forward and reverse pipetting can be performed using a liquid handling robot capable of using a manual pipette in the biomedical. Careful technique is essential as the plunger is only depressed as far as the first stop when drawing up liquid and dispensing it. This technique is especially important when pipetting small volumes pipetting forward pipetting.

Bacteria represent the most widely distributed and metabolically diverse group of organisms. It is recommended for aqueous liquids, liquids containing small amounts of detergents or proteins, and solvents. Reverse pipetting technique the reverse technique is used for pipetting solutions with a high viscosity or a tendency to foam. In forward pipetting, a desired set volume of liquid is aspirated into the disposable tip and then delivered into the. Follow these ergonomics suggestions while pipetting. Dip the tip into the solution to a depth of 1 cm, and slowly release the operating button. This technique aspirates and then dispenses the selected liquid volume. Working too quickly and pipetting at an angle are common pipetting errors. Pipetting procedures the most important aspect of pipette calibration is being consistent in your procedure and having a good cadence. Here are some very common pipetting technique errors. Steps for operating a positive displacement pipette. In air displacement pipettes, a certain volume of air remains between the piston and the liquid. This technique is most commonly used to expel an exact volume of liquid from the pipette. Adjustment altering the pipette so that the dispensed volume is within the specifications.